Interaction of Human Plasma Fibronectin with α-Elastin

抄録

Because there are contradictory reports about the interaction of plasma fibronectin with elastin, we investigated the interaction in vitro. When human plasma was applied to an α-elastin-Sepharose column at 4°C, the column-binding fraction contained fibronectin. When isolated plasma fibronectin was applied to the same column at 4°C, most of the fibronectin bound to the column and was eluted with 1M KBr. However, the binding affinity of plasma fibronectin to the α-elastin-Sepharose column was much weaker at 25°C than at 4°C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of α-elastin to fibronectin on polyvinylidene difluoride membranes using an α-elastin specific antibody. The elevation of the surface hydrophobicity of plasma fibronectin at 4°C was observed by hydrophobic chromatography, using alkyl-Sepharose columns. It seems that the binding of plasma fibronectin to α-elastin involves hydrophobic interaction, which is affected by temperature and possibly by other factors.