抄録
Seven bilirubin metabolites negative to the diazo reaction were identified in the urine of healthy persons by enzyme-linked immunosorbent assay (ELISA) using the anti-bilirubin monoclonal antibody 24G7. Two of the seven metabolites were isolated and their chemical structures were determined using fast-atom bombardment-mass spectroscopy (FAB-MS) and 1H-NMR. The two metabolites are 1, 14, 15, 17-tetrahydro-2, 7, 13-trimethyl-1, 14-dioxo-3-vinyl-16H-tripyrrin-8, 12-dipropionic acid and 1, 14, 15, 17-tetrahydro-3, 7, 13-trimethyl-1, 14-dioxo-2-vinyl-16H-tripyrrin-8, 12-dipropionic acid. They are regioisomers of each other. The two bilirubin metabolites are novel tripyrrole biocompounds and belong to a third group of bile pigments following biliverdin and bilirubin. We gave these compounds the generic names biotripyrrin-a and biotripyrrin-b, respectively.
