抄録
We examined the induction of the catalase gene (ctt1+) of fission yeast Schizosaccharomyces pombe in response to several stresses by using mutants of transcription factors (Atf1 and Pap1) and a series of deletion mutants of the ctt1+ promoter region. A transcription factor, Atf 1, and its binding site are necessary for the induction of ctt1+ by osmotic stress, UV irradiation, and heat shock. Induction by menadione treatment, which produces superoxide anion, required element A, the region from -111 to -90 (numbered with the transcription start site as +1). The factor responsible for the induction of the gene by oxidative stress via element A was identified as the transcription factor Pap 1. We also found that Atf 1 is activated by menadione treatment in pap1 mutant cells, although it is not activated by menadione treatment in pap1+ cells. The activity of catalase is not increased in pap1 cells by several stresses, despite mRNA induction, suggesting that Papl plays some role in the expression of catalase activity.
