A COLD-HEMOTOXIN, NEWLY DISCOVERED IN THE HEATED SERUM

Part III. Experiments on its Chemical Nature and on the Influence of Substances Inhibiting other Sorts of Hemolysis

抄録

1. The substance causing the cold-hemolysis in the heated normal serum can not be salted out by means of SO₄(NH₄)₂.
2. It can not be extracted with lipoid-dissolving solvants such as alcohol, aceton, ether, chloroform, etc..
3. It is always precipitated with protein when the serum is freed from protein by various methods.
4. It can be entirely adsorbed by kailin and partly adsorbed by cholesterin.
5. It is not dialysable.
6. The non-protein colloids such as glycogen, metal colloid and silicic acid, etc. have nothing to do with the cold-hemolysis.
7. Its adsorption by erythrocytes is very characteristic, that is, the erythrocytes adsorb it only when it. is in the active state in the heated serum, while the erythrocytes can not adsorb it. from the fresh unheated serum.
8. The- cold-hemolysis is markedly inhibited by the fresh unheated sera of various animals (rabbit, guinea-pig, horse, chicken, goose and man).
9. The serum-albumin can inhibit the cold-hemolysis in the same way as serum itself but the serum globulin is not endowed with such a power.
10. The cold-hemolysis is remarkably inhibited by cholesterin, but not by lecithin
. 11. It is also inhibited by egg yolk, but not by egg white.
12. The cold-hemolysis is inhibited by various proteins such as pepton, casein and trypsin, but not by ovalbumin and pepsin.
13. The bile of cattle and pig as well as the sodium salts of glycocholic- and taurocholic acid have no influence upon the cold-hemolysis.
I wish to express my indebtedness to Prof. K. Yamakami for his kind direction and encouragement during my experiments.