Abstract
1. The toxicity of urine and serum constituents are studied with bilaterally nephrectomized rabbit.
2. After the bilateral nephrectomy the pH value of blood and carbon dioxide capacity tend to decrease steadily.
3. The blood volume increases after the bilateral nephrectomy.
4. The toxicity of urea, creatine and creatine is relatively small.
5. Among the constituents of urine, only the substance, the barium salt of which is soluble in water but insoluble in alcohol, is quite toxic. This substance does not belong to alkaloid or cholin, is inactivated by hydrolysis by acid, and is dialysable through collodion membrane.
6. The minimal lethal dosis (per Kilo body weight of nephrectomized rabbit) of oxyproteic acid fraction in a narrow sense contains 0.27-0.30mg of nitrogen, while that of alloxyproteic acid fraction contains 3.28-3.30mg N. This does not mean, how-ever, that oxyproteic acid fraction proper is more toxic than alloxyproteic acid, as the nitrogen content of alloxyproteic acid far exceeds that of oxyproteic acid proper.
7. Oxyproteic acid fraction of serum has the same toxicity as that of urine.
8. The amount of oxyproteic acid in blood at 72nd hour after the bilateral nephrectomy, at the margin of uremic syndroms is 0.8-1.2mg%. This nearly corresponds to the value, which will result by the intravenous injection of 0.5mg of oxyproteic acid fraction nitrogen to the nephrectomized rabbit.
9. The estimation of serum oxyproteic acid fraction in clinical medicine is highly desirable.
I wish to express my sincere thanks to Prof. Dr. S. Kakiuchi for his kind advice throughout the course of the investigation.
