抄録
(1) The substance which is contained in the parotid gland and lowers the calcium content of blood is soluble in water, but not in alcohol, ether or acetone.
(2) The active substance indicates various protein natures, but no reduction reaction also after hydrolised it with acid.
(3) The substance loses the activity by digestion with proteinase such as trypsin or pepsin.
(4) The substance does not pass through the semipermeable membran.
(5) The substance is inactivated by heating at 75°C for 30 minutes. From these properties the active substance as the deteriorator of the calcium content might be considered as proteine like substance.
(6) The amino-groups and carboxyl-groups of the substance are necessary for the activity of the substance.
(7) The active substance is collected in the albumin fraction at the fractionation by ammonium sulfate.
(8) The water extract of ox's kidney and liver do not possess the lowering effect of the calcium content of blood, but the extract of spleen indicates the lowering effect slightly. Also in this case the active substance can be collected in the albumin fraction.
(9) The active substance from the gland and spleen differs each other serologically.
(10) The substance lowering the calcium content of blood is con-taminated also in the horse's parotid gland especially in its albumin fraction. The substances from the parotid gland of different animals differ each other serologically.
I wish to express my sincerest thanks to Prof. S. Fujimura for his kind advices and encouragement in carrying out this investigation.
