PURIFICATION OF β-GLUCOSIDASE OF ASPERGILLUS NIGER (I)
1950 年 37 巻 3 号 p. 301-308
詳細
1950 年 37 巻 3 号 p. 301-308
1. Tannin precipitation method is an excellent one for tree puri-fication of β-glucosidase of Aspergillus niger. The most active preparation obtained by this means represented the activity of 8000 times that of starting material, the dry powder of the mould.
2. pH Relationship and relative specificity did not shift in the course of purification. This suggests that this mould contains only one kind of, β-glucosidase in respect of relative specificity.
3. As this purification method permits to increase the purity of, β-glucos', dase in preference to other accompanying carbohydrases, it may be regarded as valuable one for separating β-glucosidase from them.
The cost of this research was defrayed in part by a grant from the Ministry of Education given to T. Miwa.
