Abstract
1. The normal value of pyruvic acid in the human blood was 0.85±0.02 mg./dl., and that of α-ketoglutaric acid was 0.60±0.02 mg./dI. The normal excretion of pyruvic acid in daily human urine was 21-t 1.7mg. and the concentration of the acid of daily urine was 0.91±0.11 mg./dl. The normal excretion of α-ketoglutaric acid in daily urine was 30±3.6 mg. and the concentration of the acid of daily urine was 1.10±10.12 mg./dl.
2. After exercise both blood pyruvate and α-ketoglutarate was increased, and the latter returned to the normal value faster than the former.
3. One hour after the injection of 3mg. of thiamine both pyruvate and α-ketoglutarate in blood were decreased. One hour after the injection of 10 mg. of riboflavin pyruvate in blood increased slightly and α-ketoglutarate decreased. One hour after the injection of both vitamins together, pyruvate did not show any remarkable change, whereas a-ketoglutarate was decreased.
4. The thiamine injection repressed the increase of both acids after exercise, and the riboflavin injection repressed the increase of α-ketoglutaric acid stronger than that of pyruvic acid. When both vitamins were injected together, the repression was the strongest.
The author wishes to express his sincere'thanks to Prof. N. Shimazono for his guidance and helpful advice.
