STUDIES ON THE ANTERIOR PITUITARY ACTH

IV. ACID HYDROLYSIS OF BEEF ACTH

Abstract

Beef ACTH was heated in 1 N HCl at 100° for several minutes. To 60 minutes, it retained its activity, but at 90 minutes, its activity was lost. The active peptide of these partial hydrolyzates was precipitated with 5 per cent TCA. The paper electrophoresis and N-distribution were also studied on these samples.
We wish to express our gratitude to Dr. K. Takeda for his kind guidance and encouragements through this investigation, and to Mr. A. Tanaka for his much technical assistance.