The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Search
Advanced search
OR
Browse
Search
Volume 42, Issue 1
Displaying 1-12 of 12 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • DETECTION OF XANTHURENIC ACID IN DIAZO URINE OF SEVERE TUBERCULOUS PATIENT
    KATASHI MAKINO, HITOSHI TAKAHASHI
    1955 Volume 42 Issue 1 Pages 1-2
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (101K)
  • NEW COLORIMETRIC DETERMINATION OF CALCIUM AND MAGNESIUM
    FUMIMASA YANAGISAWA
    1955 Volume 42 Issue 1 Pages 3-11
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The author presented a method for calorimetric micro-determina-tion of Ca and Mg. This direct method is very simple and direct as well as very sensitive. Total Ca and Mg as well as dialytic Ca and Mg in serum, especially the latters which are difficult to be determined by other methods, can be determined with comparative simplicity. Water hard-ness, and Ca and Mg in food can also be determined by an extension of the proposed method.
    View full abstract
    Download PDF (624K)
  • THE INCORPORATION OF PHOSPHORUS FROM P32-LABELED COCARBOXYLASE INTO ADENOSINE TRIPHOSPHATE BY RAT LIVER HOMOGENATE AND CYCLOPHORASE SYSTEM
    KIKUO OGATA, HIROYOSHI NOHARA, TOMI MORITA, KAZUO KAWAI
    1955 Volume 42 Issue 1 Pages 13-25
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. Rapid incorporation of P32-cocarboxylase into the terminal P of ATP was observed in the tissue homogenate or especially in the liver cyclophorase system in the presence of α-ketoglutarate or pyruvate.
    2. From the experiment using the liver cyclophorase system it was especially indicated that there might be a direct route (not via inorganic P) of phosphate transfer from cocarboxylase to the terminal P of ATP in the presence of α-ketoglutarate or pyruvate.
    3. A mechanism assuming that a binding form of cocarboxylase (lipothiamide pyrophosphate) might be formed as an intermediate, may be forwarded from the study in which various amounts of cocarboxylase was incubated with cyclophorase system in the presence of α-ketoglutar-ate.
    4. Approximate rate of the incorporation of P32 of P32-cocarboxylase into ATP was compared with that of inorganic P32 in the presence of NaF which prevented almost completely the release of inorganic P from cocarboxylase.
    5. The rate of the incorporation of P32-cocarboxylase into ATP was not inhibited by 2, 4-dinitrophenol.
    6. The added cocarboxylase appeared as labile P in the liver cyclophorase system.
    View full abstract
    Download PDF (867K)
  • THIAMINE UPTAKE BY YEAST CELLS
    SUZUOKI ZIRO
    1955 Volume 42 Issue 1 Pages 27-39
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    In the presence of glucose baker's yeast uptook thiamine after 15 minutes lag period both under aerobic and anaerobic conditions. The preincubation with glucose enabled the cells to uptake thiamine without lag phase even in the absence of glucose. The effects of several metabolic substrates or inhibitors were investigated under various conditions. It was concluded that yeast uptook thiamine according to a typical active transport involving the energy-generating and -transferring systems. Thiol-type'derivatives of thiamine were not uptaken under the same conditions.
    View full abstract
    Download PDF (676K)
  • BIOCHEMICAL STUDIES ON SULFATE-REDUCING BACTERIA
    IV. REDUCTION OF THIOSULFATE BY CELL-FREE EXTRACT
    MAKOTO ISHIMOTO, JIRO KOYAMA, YUTAKA NAGAI
    1955 Volume 42 Issue 1 Pages 41-53
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Cell-free extracts from cells of sulfate-reducing bacteria ground with alumina has an ability to reduce thiosulfate to sulfite and sulfide and then to sulfide slowly with an equivalent amount of molecular hydrogen in the presence of methyl viologen as an intermediary carrier. This reaction proceeded in two phases, hydrogenation of the dye by hydrogenase and thiosulfate reduction with the reduced dye catalysed by a specific enzyme. The reduced methyl viologen was found to reduce thiosulfate in presence of the dialysed extract. The conditions of the reactions were investigated.
    The authors wish to express their gratitude for kind guidance made by Dr. T. Soda and for the valuable advice rendered by Dr. H. Tamiya and Dr. S. Akabori, and wish to thank Dr. A. Takamiya for giving us methyl viologen and Dr. Y. Oda for gifting hydrogenase preparation of Azotobacter. The expences of the work were aided partly by the Scientific Research funds from the Ministry of Education.
    View full abstract
    Download PDF (776K)
  • STUDIES ON TAKA-AMYLASE A
    III. CARBOHYDRATE COMPONENT IN TAKA-AMYLASE A
    HIDESABURO HANAFUSA, TOKUJI IKENAKA, SHIRO AKABORI
    1955 Volume 42 Issue 1 Pages 55-62
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Taka-amylase A obtained from “Takadiastase Sankyo” contains 8 moles of mannose, 1 mole of xylose and 2 moles of hexosarnine as in-tegral constituent of the molecule. Taka-amylase obtained from a synthetic culture solutions the least amount of carbohydrate. Carbo-hydrate component seems to be not essential to amylase activity.
    View full abstract
    Download PDF (443K)
  • STUDIES ON XANTHURENIC ACID
    XI. RESEARCH ON THE MECHANISM OF INITIAL HYPERGLYCEMIA CAUSED BY XANTHURENIC ACID
    YAHITO KOTAKEJR, TOSHIRO INADA, YUICHI MATSUMURA
    1955 Volume 42 Issue 1 Pages 63-68
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. On albino rats with their hypophysis or suprarenal gland extirpated the initial hyperglycemia by xanthurenic acid did not appear. In these cases the liver glycogen contents were found invariably reduced by half as compared with normal ones.
    2. Following the injection of xanthurenic acid, the ascorbic acid amount in the suprarenal gland was found reduced just as in the case of epinephrine injection.
    3. Hyperglycemia due to epinephrine was inhibited by benzyl-imidazolin, but not that due to xanthurenic acid.
    4. These experimental facts lead us to believe that the initial hyperglycemia caused by the injection of xanthurenic acid is due partly to hormons of suprarenal glands and hypophysis, the production of which being enhanced by the injected acid, and partly to its direct action on liver tissue.
    View full abstract
    Download PDF (380K)
  • ON THE MECHANISM OF THE ACTIVATION OF α-CHYMOTRYPSINOGEN TO α-CHYMOTRYPSIN
    II. THE CHANGES IN AMINO-TERMINAL AMINO ACIDS OF α-CHYMOTRYPSINOGEN DURING THE ACTIVATION BY TRYPSIN
    NAOKAZU SAKOTA
    1955 Volume 42 Issue 1 Pages 69-75
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. The changes in N-terminal groups of α-chymotrypsinogen during the activation has been investigated, and seven kinds of N-terminal amino acids were detected for the most part at the initial stage of the activation process.
    2. The activation process of α-chymotrypsinogen to α-chymotrypsin by trypsin involves the opening of cyclic polypeptide chains of the zymo-gen, yielding alanine at first and then isoleucine as amino-terminal groups of α-chymotrypsin, and it may be accompanied with the liberation of peptides, transpeptidation, secondary structural modification, and so on.
    The author wishes to thank Professor Shiro Akabori for his guidance throughout of this work, also Mr. Yoshimi Okada for his valuable technical assistance.
    View full abstract
    Download PDF (393K)
  • THE SYNTHESIS OF STERO-BILE ACIDS
    XII. THE PARTIAL SYNTHESIS OF BISNORSTEROCHOLIC ACID BY KOLBE ELECTROLYTIC REACTION
    TARO KAZUNO, AKIMICHI MORI, TADASHI GOTO
    1955 Volume 42 Issue 1 Pages 77-80
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (200K)
  • STUDIES ON THE ANTERIOR PITUITARY ACTH
    III. CHROMATOGRAPHY OF BEEF ACTH ON ION EXCHANGE RESIN COLUMN
    HIDEO OTSUKA, TOKUJI KIMURA
    1955 Volume 42 Issue 1 Pages 81-85
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Beef ACTH was adsorbed on Amberlite IRC-50 at pH 7.8 and could be eluted with acetic acid, but foric acid destroyed its hormonal ac-tivity. Elution with acetic acid increased hormonal activity about 20 folds.
    We wish to express our gratitude to Prof. S. Akabori, Osaka University and Dr. K. Takeda for their kind guidance and encouragements through this work, and to Mr. A. Tanaka for his much technical assistance.
    View full abstract
    Download PDF (244K)
  • STUDIES ON THE ANTERIOR PITUITARY ACTH
    IV. ACID HYDROLYSIS OF BEEF ACTH
    HIDEO OTSUKA, NOBUO YOSHIDA
    1955 Volume 42 Issue 1 Pages 87-91
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Beef ACTH was heated in 1 N HCl at 100° for several minutes. To 60 minutes, it retained its activity, but at 90 minutes, its activity was lost. The active peptide of these partial hydrolyzates was precipitated with 5 per cent TCA. The paper electrophoresis and N-distribution were also studied on these samples.
    We wish to express our gratitude to Dr. K. Takeda for his kind guidance and encouragements through this investigation, and to Mr. A. Tanaka for his much technical assistance.
    View full abstract
    Download PDF (310K)
  • THE ACTIVE CENTER OF THIAMINE
    YOKO TAKEUCHI, KYUICHIRO OKUDA, SHOHEI HAYAKAWA, SHUNZI MIZUHARA
    1955 Volume 42 Issue 1 Pages 93-97
    Published: 1955
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Cysteine, thioglycolic acid, thioglycolic acid-pyruvic acid addition compound, thiophenol, thiophenol-pyruvic acid addition compound, and O, S-diacetylthiamine were tested of their ability of acyloin condensation, and it was found that these sulfhydryl compounds could not serve as the catalysts for the aceloin condensation. However, sodium cyanide can catalyze the acetoin formation from pyruvate and acetaldehyde.
    This work was done by a fund of the Vitamin B Research Committee.
    View full abstract
    Download PDF (254K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top