Abstract
The influences of copper ion on the DPN-reduction of highly purified alcohol dehydrogenase extracted from bakers' yeast and of crystalline glyceraldehyde phosphate dehydrogenase extracted from rabbit muscle were observed in the presence and absence of various additional sub-stances in the reaction mixture. The neutralizing action of the additions tested against the copper inhibition was estimated as follows: Aspartate and malate were strong, citrate medium, and succinate, tartarate, acetate and glucose weak.
When some of the substances described above were added to the culture medium, Mycobacterium tuberculosis avium could grow well in the presence of copper in a concentration enough to inhibit the growth in the medium without the additions. This phenomenon did not follow the essential increase in the copper-resistant ability. The grade of the neutralizing power was follows: Aspartate, oxalate, citrate, ammonium citrate, glucose, and mannitol were strong, glutamate and malonate medium, acetate, malate, succinate, arabic gum, and ammonium sulfate weak. Namely, the influences of glucose and malate ran counter to each other in the two experiments. On the basis of such differences, possible explanation on the increase in the copper-resistant ability of the microörganisms was discussed.
This work was supported in part by the Grant in Aid for Experimental Scientific Research from the Ministry of Education.
