STUDIES ON CONJUGATION OF S³⁵-SULFATE WITH PHENOLIC COMPOUNDS

III. METABOLISM OF p-CRESOL, p-HYDROXYBENZALDEHYDE AND p-HYDROXYBENZOIC ACID IN RAT LIVER

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1. A study has been made of the sulfate conjugation of p-hydroxy-benzoic acid, p-hydroxybenzaldehyde and p-cresol in rat liver by the aid of S³⁵-sulfate. Paper chromatography and radioautography has been used to examine the conjugates.
2. There has been found various sulfate conjugates related to the above substances.
3. It has been proved that p-cresol is oxidized to p-hydroxybenzoic acid through p-hydroxybenzaldehyde in the rat liver.
There seemed to exist another intermediate, p-hydroxybenzyl alco-hol, as a precursor of p-hydroxybenzaldehyde.
4. The steps from -CH₃ to a substance supposed to be -CH₂OH and from -CHO to -COON were shown to proceed even in the supernatant of the rat liver homogenate.
5. The occurrence of the sulfate conjugate of 3, 4-dihydroxybenzoic acid, from p-hydroxybenzoic acid with rat liver slices, was concluded, from its Rf value, though its amount was meagre.
6. Another sulfate conjugate has been found in the metabolite of p-hydroxybenzoic acid with liver slices, and this will probably be the sulfate conjugate of p-hydroxyhippuric acid