THE QUANTITATIVE DETERMINATION OF AMINO ACIDS BY PAPER CHROMATOGRAPHY
1956 年 43 巻 4 号 p. 523-529
詳細
1956 年 43 巻 4 号 p. 523-529
A method has been described for accurate estimation of amino acids on paper chromatograms.
It consists of, a) visual comparisons of series of spots obtained from dilutions of unknown and standard solutions to give an estimate of the unknown in terms of standard to within 5 per cent, and b) a more exact comparison of closely similar dilutions of standard and unknown in terms of a special technique of measurement of spot size.
The coefficient of variation of individual estimations by this method is ±1.24 per cent.
