ENZYMATIC DETERMINATION OF ADENOSINE TRIPHOSPHATE USING YEAST HEXOKINASE
1958 年 45 巻 2 号 p. 65-72
詳細
1958 年 45 巻 2 号 p. 65-72
1. For the determination of ATP, a simple reaction of hexokinase prepared from yeast was employed by measuring diminution of acid-labile phosphorus. Compared with two other methods, manometric and spectrophotometric, using hexokinase, the present method is easy to use.
3. Illustrative examples are given of analysis of ATP in pure solution and in extracts from physiological materials. The results obtained from these experiments, especially in the latter case, make it quite clear that the acid-labile phosphorus is not the representative of ATP, but phosphorus incorporated into hexose is compatible with the content of ATP.
The expenses of the present work were aided partly by the Scientific Research Grant given to Okunuki from the Ministry of Education.
The authors wish to express many thanks to the Oriental Yeast Ltd. for their kind supply of the pressed yeast.
