STUDIES ON EGG YOLK PROTEINS

V. ELECTROPHORETIC AND ULTRACENTRIFUGAL INVESTIGATIONS ON THE HOMOGENEITIES AND SOME PROPERTIES OF α- AND β-LIPOVITELLIN

Abstract

1. Both α- and β-lipovitellin were homogeneous elect rophoretically in the pH range 6.8 to 9.9 and 4.9 to 10.5, respectively, whereas at pH 4.2 both were heterogeneous. Ultracentrifugal analysis at pH 9.8 and μ 0.15 indicated the heterogeneities of both preparations.
2. From the mobility curve, the isoelectric point of β-lipovitellin was pH 5.9 at an ionic strength of 0.30.
3. From the variation of mobility of β-lipovitellin with buffer composi-tion, it was pointed out that the divalent anions such as carbonate and sulfate had the binding ability to β-lipovitellin, but not monovalent ions such as ammonium and chloride.
4. The mobility of β-lipovitellin depended on the inoic strength of buffer. There was a marked decrease in the mobility of β-lipovitellin with increase in ionic strength.
5. Some informations on the solubility property of β-lipovitellin in the pH range from 4.0 to 10.0 were obtained.