1959 Volume 46 Issue 4 Pages 425-431
1. The hydrolysis by crystalline Taka-amylase A of α-phenylmaltoside and p-nitrophenyl-α-maltoside was confirmed and the maltosidasc activity of Taka-amylase A was found to be an intrinsic property of this enzyme.
2. The preparation and properties of crystalline α-phenylmaltoside and the synthesis of p-nitrophenyl-α-maltoside were described.
3. The method of determination of Taka-amylase A activity using α-phenylmaltoside and p-nitrophenyl-α-maltoside was described, and the maltosidase unit was proposed as a new measure of Taka-amylase activity.
The authors wish to express their gratitude to Mr. A. Tsugita, T. Matshsim a, K. Yamamoto and Miss H. Toda for their helpful advice through this investigation, and also to Sankyo Co., Ltd. for their kind supply of “Takadiastase Sankyo”.