Abstract
1. Cytochromes of Pseudomonas-P were studied by the opal glass method. Pseudomonas-P had cytochromes 553 and 560. Cytochrome 553 was extracted and partially purified.
2. Cytochrome content in Pseudomonas-P varied remarkably in response to the growth phase and as well as to the growth medium.
3. The distribution of cytochromes on cellular components of Pseudomonas-P was examined. Both of cytochromes 553 and 560 were the most abundant in SP (small particle) fraction.
4. The incorporation of racioactive iron into growing cells of Pseudomonas-P and the distribution of radioactivity taken up on the cellular components were examined. The results were discussed in relation to the observations on cytochrome formation and localization.
5. The incorporation of radioactive iron into heme by the washed cells, the disrupted cells or the cellular components of Pseudomonas-P was examined. Of the cellular components, LP (large particle) fraction was the most active in the iron incorporation.
The authors express their thanks to Prof. S. Akabori and Prof. H. Yoshikawa for their interests during the course of this work. Thanks are also due to Dr. K. Shibata for his aid in the application of his opal glass method to this work.
