ON THE TRANSPHOSPHORYLATION REACTIONS CATALYZED BY GLUCOSE-1-PHOSPHATE PHOSPHOTRANSFERASE OF ESCHERICHIA COLI

II. ENZYMATIC PHOSPHORYLATION OF NUCLEOSIDES

Abstract

1. It was shown that G-1-P phosphotransferase of E. coli catalyzed the transphosphorylation from G-1-P to nucleosides, synthesizing the corresponding nucleotides.
2. The experimental results using adenosine, indicated that the enzyme specifically phosphorylated the 5'-position of sugar moiety of nucleoside.
3. A possible role of G-1-P phosphotransferase in the biosynthesis of nucleotides was discussed.