STUDIES ON THE RESPIRATORY ENZYME SYSTEMS OF PLANTS

I. ENZYMATIC OXIDATION OF α-NAPHTHYLAMINE IN RICE PLANT ROOT

抄録

1. The partially purified peroxidase from leaf and root of rice plant respectively and crystalline peroxidase named JRP-a from Japanese raddish can oxidize α-NA with H₂O₂. The oxidized product of α-NA was a dark violet colored substance, and was soluble scarcely in water, slightly in ethyl ether and very easily in ethylacetate. β-NA was also oxidized by this enzyme system as well as α-NA.
2. The peroxidatic oxidation of α-NA needs one mole of H₂O₂ per one mole of α-NA.
3. The α-NA-oxidizing activity which seems to be available for the method of diagnosing of the root activity in agronomy, may represent the rate of the production and supply of H₂O₂ or other peroxides in tissue to be measured.
I wish to express my thanks to Dr. Y. Morita for supplying the crystalline pero-xidase used in this study, and also to Dr. I. Yamazaki for his helpful suggestions.