Abstract
The large fractions 4 and 5, described in the first report, obtained after the first cellulose powder column chromatography of the N-acetylated 2-amino sugar-containing compounds which were separated from the acid hydrolyzate of the hydrazinolyzed product of blood group A mucopolysaccharide of hog gastric mucus, were further fractionated on cellulose powder columns and char-coal columns.
Of the fractions, a disaccharide (Oligosaccharide III) and a trisaccharide (Oligosaccharide IV) were isolated.
On the basis of the analytical data as well as the values of sodium metaperiodate oxidation before and after reduction with sodium borohydride and their physical properties, the structures of these compounds have been firmly established: Oligosaccharide III is O-β-2-acetamido-2-deoxy-D-glucopyrano-syl-(1→6)-D-galactose and Oligosaccharide IV is O-β-2-acetamido-2-deoxy-D-glucopyranosyl-(1→3)-[O-β-2-acetamido-2-deoxy-D-glucopyra-nosyl-(1→6)-]-D-galactose.
This work was aided by a grant for scientific researches from the Ministry of Education.
