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Volume 51 (1962)
- Issue 6 Pages 385-
- Issue 5 Pages 313-
- Issue 4 Pages 233-
- Issue 3 Pages 169-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 51, Issue 2
Displaying 1-10 of 10 articles from this issue
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II. Further Purification and Amino Acid Composition of Ribonuclease T1KENJI TAKAHASHI1962Volume 51Issue 2 Pages 95-108
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. An improved method for preparation of pure RNase T1 was described.
2. The purified enzyme was demonstrated to be homogeneous by moving boundary electropdoresis and some other criteria, and the isoelectric point was determined to lie around pH 2.9.
3. The enzyme was shown to be free from sugar and the nitrogen content was determined to be 16.5 per cent.
4. The amino acid composition of the enzyme was determined. The corrected analytical values accounted quantitatively for the weight and the nitrogen content of the enzyme. The analyses indicated the following 105 amino acid residues in the enzyme molecule (mol. wt. 11, 127): Asp15 Thr6 Ser17 Glu10 Pro4 Gly12 Ala7 (Cys-)4 Val7 Ileu2 Leu3 Tyr8 Phe4 Lys1 His3 Arg1 Try1(-CONH2)11.
The author wishes to express his sincere thanks to Prof. F. Egami for his guidance and encouragement during this work. He is grateful to Prof. T. Ando and Dr. K. Iwai for affording him facilities for using the Beckman-Spinco automatic amino acid analyzer and to Mrs. Y. Misaw a for operation of the Spinco electrophoresis apparatus. He also expresses his gratitude to Sankyo Co. Ltd. for the kind gift of “Takadiastase Sankyo”. The expense of this study was defrayed in part by a grant from the Ministry of Education.View full abstractDownload PDF (3090K) -
Anaysis and ReleaseISAMU SHIIO, KIKUKO NARUI, NORIKO YAHABA, MASAHIRO TAKAHASHI1962Volume 51Issue 2 Pages 109-111
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Amino acid compositions of free intracellular pool and external medium of Brevibacterium fiavum No. 2247 grown in biotinpoor and -rich media were analysed microbiologically. Of 18 amino acids, the major components of internal amino acid pool were glutamic acid, alanine, valine, lysine, and serine; and the major components of external medium were glutamic acid, aspartic acid, and alanine under biotin-poor condition, and alanine alone under biotin-rich condition.
2. Under biotin-poor condition, amino acid contents of internal pool were much less than those under biotin-rich condition and all the amino acids decreased strikingly on washing with a buffer, while under the latter condition acidic and basic amino acids did not decrease significantly on washing. These behaviors seem to be correlated with the extracellular accumulation of amino acids in B. flavum.
The authors are indebted to Dr. H. Oeda and Dr. Y. Tsuchiya of our laboratory for their interest and discussion during the course of this work.View full abstractDownload PDF (203K) -
XXXVII. Formation of Bile Sterols from Cholesterol in Bull Frog, Rana CatesbianaTAKAKO MASUI1962Volume 51Issue 2 Pages 112-118
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSFrom bile of bull frog injected with cholesterol-[C14-4], 3α, 7α, 12α, 26-tetrahydroxy-Δ23bishomocholene-[C14-4], 3α, 7α, 12α-trihydroxy-Δ24-homocholene-[C14-4] and a radio-active new bile sterol of m. p. 182-3°C were isolated, and it was confirmed that these sterols were biosynthesized from cholesterol in bull frog.
The author wishes to express her deep gratitude to Prof. T. Kazuno for his kind advice throughout this work.View full abstractDownload PDF (312K) -
XLII. Chemical Structure of TrihydroxyhomocholeneTAKAKO MASUI, TARO KAZUNO1962Volume 51Issue 2 Pages 119-123
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSDownload PDF (244K) -
I. Ceramide-Oligohexosides of Human, Equine and Bovine SpleensAKIRA MAKITA, TAMIO YAMAKAWA1962Volume 51Issue 2 Pages 124-133
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSGlycolipid fractions from human, equine and bovine spleens were separated by use of column chromatography on silicic acid. Among them, ceramide-oligohexosides were purified on Florisil column. From each spleen, glucocerebroside and ceramide-dihexoside containing both glucose and galactose were obtained. Moreover, in bovine spleen, ceramide-trihexoside was found.View full abstractDownload PDF (1328K) -
I. Catabolism of 2, 3-Diketo-L-Gulonic AcidYASUO KAGAWA1962Volume 51Issue 2 Pages 134-144
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Potassium 2, 3-diketo-L-gulonate was synthesized and its metabolism in animal tissues was studied to clarify the catabolism of ascorbic acid.
2. Metabolic products of diketogulonate in vitro were confirmed as L-lyxonic acid and L-xylonic acid in the presence of decarboxylase of diketogulonate in neutral condition, and as L-threonic acid and oxalic acid in aerobic alkaline condition. All three aldonic acids were isolated and crystallized in the form of benzimidazole derivatives. Formation of the same metabolic products was also detected in vivo.
3. The enzyme, 2, 3-diketoaldonate decarboxylase, which catalysed the decarboxyla-tion of diketogulonic acid and other diketoaldonic acids, was purified about 50 fold from rat liver supernatant. Properties of the decarboxylase, including substrate affinity constant and stoichiometry were described. The enzyme is distributed in liver and kidney supernatant fraction of animals including primates.
The author wishes to express gratitude to Prof. N. Shimazono for his interest and encouragement.View full abstractDownload PDF (1562K) -
II. Isolation and Characterization of O-β-2-Acetamido-2-deoxy-D-glucopyranosyl-(1→6)-D-galactose and O-β-2-Acetamido-2-deoxy-D-glucopyranosyl-(1→3)ZENSAKU YOSIZAWA1962Volume 51Issue 2 Pages 145-154
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSThe large fractions 4 and 5, described in the first report, obtained after the first cellulose powder column chromatography of the N-acetylated 2-amino sugar-containing compounds which were separated from the acid hydrolyzate of the hydrazinolyzed product of blood group A mucopolysaccharide of hog gastric mucus, were further fractionated on cellulose powder columns and char-coal columns.
Of the fractions, a disaccharide (Oligosaccharide III) and a trisaccharide (Oligosaccharide IV) were isolated.
On the basis of the analytical data as well as the values of sodium metaperiodate oxidation before and after reduction with sodium borohydride and their physical properties, the structures of these compounds have been firmly established: Oligosaccharide III is O-β-2-acetamido-2-deoxy-D-glucopyrano-syl-(1→6)-D-galactose and Oligosaccharide IV is O-β-2-acetamido-2-deoxy-D-glucopyranosyl-(1→3)-[O-β-2-acetamido-2-deoxy-D-glucopyra-nosyl-(1→6)-]-D-galactose.
This work was aided by a grant for scientific researches from the Ministry of Education.View full abstractDownload PDF (2265K) -
ZENSAKU YOSIZAWA, TOKUTARO SATO1962Volume 51Issue 2 Pages 155-161
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Sodium chondroitin sulfate A and sodium hyaluronate were subjected to hydrazinolysis and the non-dialyzable and dialyzable products of the hydrazinolyzates were examined.
2. Only small amounts of hydrazinolyzed products of these acid mucopolysaccharides passed through cellophane membranes. By the hydrazinolysis, the decomposition of glucuronic acid was scarce whereas approximately 10 per cent of total hexosamines were degraded.
3. Besides small amounts of some unknown degradation products, small amounts of 2, 5-anhydrohexoses and pentoses (lyxose from the chondroitin sulfate A and arabinose from the hyaluronate) were found in the hydrazinolyzed products. These sugars were thought to be intermediates in the degradation reaction of N-acetylhexosamines by the hydrazinolysis.
4. The per cent N-deacetylation by the hydrazinolysis of N-acetylgalactosamine in sodium chondroitin sulfate A and of N-acetyl-glucosamine in sodium hyaluronate amounted to 28.2 per cent and 22.9 per cent, respectively. Approximately 36 per cent of the N-deacetylated hexosamines in the non-dialyzable products of the hydrazinolyzates could be determined as 2, 5-anhydrohexoses by the direct deamination with nitrous acid.
5. Sulfates in the sodium chondroitin sulfate A remained in the non-dialyzable product of the hydrazinolyzate.View full abstractDownload PDF (436K) -
ZENSAKU YOSIZAWA, KIYOKO INO, YOSHIO FUJIWARA1962Volume 51Issue 2 Pages 162-166
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Partially N-deacetylated mucopolysac-charide obtained by the hydrazinolysis of blood group A mucopolysaccharide of hog gastric mucus was N-sulfated with pyridinesulfur trioxide and aqueous sodium hydroxide.
2. N-Sulfated partially N-deacetylated mucopolysaccharide was O-sulfated with sulfan in liquid sulfur dioxide.
3. The product was revealed to contain approximately 1.29 sulfate groups per monosaccharide unit. These sulfate groups were found to be bound to all of the amino groups of the N-deacetylated hexosamines and partly to the axial and equatorial hydroxy groups in the mucopolysaccharide on the basis of the analytical data as well as the absorptions of infrared ray.
4. The product showed a high lipemia clearing activity in in vivo assay, but practically no anticoagulant activity in in vitro test.View full abstractDownload PDF (289K) -
KYUSHIRO FUJII, SUMITO MIZOTA, TAKASHI TAKAMA, SHO MIYAMOTO, TAKASHI O ...1962Volume 51Issue 2 Pages 167-168
Published: February 25, 1962
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSDownload PDF (104K)
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