Effect of Biotin on the Bacterial Formation of Glutamic Acid
II. Metabolism of Glucose
1962 年 52 巻 2 号 p. 108-116
詳細
1962 年 52 巻 2 号 p. 108-116
1. Glucose was completely oxidized to form 6 moles of carbon dioxide in the washed cells of Brevibacterium flavum No. 2247, grown in a biotin-rich medium, while, in the cells grown in a biotin-poor medium, 0.5-0.7 moles of α-ketoglutarate as well as carbon dioxide were formed as the end product. The end products in the aerobic metabolism of pyruvate were similar as in that of glucose, respectively.
2. Any significant effect of biotin concentration in the culture medium was not observed on the formation of pyruvate and citrate by the washed cells, on the metabolism of citrate in the detergent-treated cells, and also on the various enzyme activities of cell extracts.
3. A considerable amount of L-glutamate was formed from glucose also in the cells grown in a biotin-rich medium if some detergents were added to the reaction mixture; the detergents had been known to make these cells permeable to glutamate.
4. These results support strongly the “permeability hypothesis” that has been previously proposed with regard to the effect of biotin on the extracellular formation of glutamate.
The present authors are indebted to Dr. H. Oeda and Dr. Y. Tsuchiya of our laboratory for their interest and encouragement during the course of this work.
