抄録
1. Properties of trans- and cis-benzenegly-col have been studied by paper chromatography, paper electrophoresis, colour reactions and sulphate conjugating technique. The diols produce phenylsulphate, presumably through dehydration of the conjugate of them with sulphate in the supernatant of animal liver homogenate, and catechol sulphate. trans-Benzeneglycol is more easily dehydrogenated to produce catechol sulphate than the cis-diol.
2. A substance indistinguishable chromatographically and electrophoretically from traps-benzeneglycol has been found in the urine of rabbits dosed with benzene.
3. A substance, which is a glucuronide, has been found. It produces a substance indistinguishable from trans-benzeneglycol by the treatment with β-glucuronidase and turns to a stable glucuronide producing phenol by acid dydrolysis.
4. The phenylmercapturic acid precursor has been shown by paper chromatography. Incubating benzene with animal livers, a substance, which produces the phenylmercapturic acid precursor in animal kidney slices, has been found.
5. These observations have been discussed in relation to the epoxide formation in the course of the metabolism of benzene in animal body.
Many thanks are due to Prof. M. Nakajima and Dr. N. Kurihara of Kyoto University for their kind giving of benzeneglycols, and to Prof. E. Boyland and Dr. P. Sims of the Chester Beatty Research Institute, London, for their kind teaching and giving of chemicals for the experiment.
