抄録
A cell-free extract of the mucous gland of Charonia lampas was demonstrated to incorporate C14-glucose into charoninsulfuric acids in the presence of ATP. ATP was required absolutely and magnesium ions were also necessary for glucose incorporating activity of the cell-free extract. No requirement for UTP was recognized. ATP could not be replaced by AMP or UTP. ATP was converted to AMP during incubation. The addition of external sulfate or charoninsulfuric acids had no effect on the incorporating activity. The incorporation of C14-glucose preceded into S-poor charoninsulfuric acid than into S-rich one. Almost all incorporating activity of the cell-free extract was found in the supernatant after centrifuged at 6000 r.p.m.
The author, wishes to express his gratitude to Prof. F. Egami of Tokyo University for his useful advice. This research was supported by grants from the Scientiffc Research Fund of the Ministry of Education and Seikagaku-kenkyusho Ltd. Some of the experiments were carried out in the Marine Biological Station of Nagoya University.
