Abstract
1. The chromatographic examination of crystalline Taka-amylase A on the DEAE-cellulose column has shown the presence of small amount of inactive impurities. Further purification of the crystalline TAA was attempted using DEAE-cellulose column.
2. Basing on the chromatographic behavior of TAA, a simplified chromatographic preparation of homogeneous enzyme was made.
3. Direct separation of enzymes from the “Takadiastase” extract was carried out by the aid of chromatography on DEAF-cellu-lose. Alkailne protease, Taka-maltase and Taka-amylase A could be separated direct-ly.
The authors wish to express their thanks to Prof. K. Narita and Dr. T. Ikenaka for their kind guidance and valuable discussions through this investigation and also to Sankyo Co., Ltd. for their kind supply of “Takadiastase Sankyo”.
