Intracellular Transfer of Nucleic Acids

III. Fate of Nucleic Acids in Rat Ascites Hepatoma Cells

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1. C¹⁴-AICA was administered intraperi-toneally to the rats bearing ascites hepatoma, AH 130 or AH 7974, after 2, 4 and 8 hours. Hepatoma cells were collected and homogeni-zed. The cytoplasmic supernatant and the supernatant of sonicated nuclei were analyzed by DEAE cellulose column chromatography.
2. The elution patterns were essentially identical to those of normal rat livers except for the high peak at the cytoplasmic s-RNA fraction.
3. The metabolic turnover of RNA is approximately twofold faster in hepatoma cells than in normal liver cells.
4. Chromomycin A₃ inhibits specifically the incorporation of a labeled precursor into an s-RNA fraction.
This investigation was supported by grants from the National Institute of Health, U.S.A. (CV-5869 CY) and the Ministry of Education, Japan.