Abstract
1. The absorption spectra of the reduced and oxidized forms of the respiratory enzyme were calculated by correlating quantitatively the photochemical action spectrum with the difference spectra of cytochrome oxidase (a3++CO-a3++, and a3++CO-a3+++).
2. A quantitative correlation was achieved by taking advantage of the fact that the spectra of the cytochrome a3++-carbon mono-xide complex, cytochrome a+++, and cyto-chrome oxidase (a++++a3++CO) had quite different ratios of absorption at 590mμ to that at 550mμ. This method of calculation gave the reduced spectrum more easily than the previous method.
3. The resulting spectra of the reduced and oxidized forms of the respiratory enzyme were in reasonable agreement with the results calculated previously.
4. The spectra of oxidized and reduced cytochrome a were also calculated from the spectra of the cytochrome oxidase-carbon monoxide complex and the photochemical action spectrum on the basis of the same principle.
The author expresses his cordial thanks to Prof. N. Shimazono for his kind advice and encouragement. He also wishes to thank Prof. K. Shibata of the Tokyo Institute of Technology for his valuable advice.
