Abstract
1. For further studies of intracellular localization of hexosamine and sialic acid, the mitochondrial and the microsomal frac-tions of rabbit liver cells were submitted to purification and subfractionation. Mito-chondria was purified with particular atten-tion paid for microsomal contamination. Microsome was fractionated into smooth- and rough-surfaced microsomes.
2. It could be concluded that mitoch-ondria contained definite amounts of hexo-samine (1.7-2.0μg. per mg. protein) and sialic acid (1.3-1.6μg. per mg. protein).
3. Microsomal hexosamine and sialic acid were found to be concentrated in the smooth-surfaced microsome rather than in the rough-surfaced microsome.
4. The ratios of hexosamine: sialic acid in mitochondria and the smooth-surfaced microsome were similar for each other and also comparable to that found for nucleus.
