Abstract
1. The enzymatic methods were described for determining systematically the levels of glycolytic intermediates and nucleotides in the red blood cells, which were deproteinized immediately after removal from the body.
2. The contents of the metabolites in red blood cells of human males were found to be fairly constant, if the blood was subjected to analysis immediately after sampling from a vein and without further manipulation.
3. The sources of errors in the enzymic determination of glycolytic intermediates in red blood cells were discussed.
