Photooxidation of Adenine and Its Nucleotides in the Presence of Riboflavin
I. Photooxidation of NAD and NADP
1966 年 59 巻 5 号 p. 433-437
詳細
1966 年 59 巻 5 号 p. 433-437
The photochemical inactivation of NAD and NADP by visible light in the presence of riboflavin was investigated.
1. The photochemical inactivation of the nucleotides proceeded with irradiation time.
2. The photochemical inactivation of NADP proceeded in the same way as that of NAD.
3. Riboflavin was not required in stoichiometric amounts.
4. Optimum pH of photochemical in-activation of the nucleotides was found to be around pH 4.0.
5. Oxygen is necessary for the photo-chemical inactivation of the nucleotides.
6. The photochemical inactivation of the nucleotides in the presence of methylene blue does not occur.
The authors wish to express their thanks to Miss K. Kobayashi for her assistance in the present study.
