Heterogeneous Incorporation of C14-Amino Acids into Guinea Pig 7S γ-Globulins
1966 年 60 巻 1 号 p. 27-36
詳細
1966 年 60 巻 1 号 p. 27-36
The heterogeneity of guinea pig 7S γ-globulins and the rates of incorporation of C14-amino acids into electrophoretically dif-ferent 7S γ-globulin have been investigated.
1. Experiments in vivo showed that C14-amino acids were incorporated into electro-phoretically different fractions of guinea pig 7S r-globulins at different rates, indicating the presence of three different types of 7S γ-globulins which were referred to as slow 7S γ2-, fast 7S γ2- and 7S γ1-globulins.
2. The experiments in vitro with various tissues showed that the spleen, lymph nodes, bone marrow and lung incorporated actively C14-amino acids into both 7S γ1- and 7S γ2-globulins.
3. Furthermore, the expeviments in vitro revealed that C14-amino acids were incorpo-rated into different fractions of 7S γ2-globulin at different rates, depending on whether spleen, bone marrow, lymph nodes or lung was used.
4. From these results, it was suggested that guinea pig 7S γ1- and 7 S γ2-globulins were synthesized by different types of plasma cells and also that electrophoretically different 7S γ2-globulins were synthesized by different cells which were unevenly distributed in the various tissues.
5. Immunological examinations of guinea pig 7S γ-globulins for antigenicity were also performed and a result suggesting three sub-classes of 7S γ-globulin (slow 7S γ2-, fast 7S γ2- and 7S γ1-globulins) was obtained.
The present authors wish to express their sincere thanks to Prof. I. Yamashina for his encouragement during this work.
