Abstract
To study the heterogeneity of guinea pig 7S γ-antibody, the incorporation of C14-amino acids into these proteins was studied using various lymphoid tissues from guinea pigs which were hyperimmunized with ovalbumin.
1. The spleen, bone marrow, mesenteric lymph nodes, granuloma at the site of injec-tion and lung actively incorporated C14-amino acids into both 7S γ1- and 7S γ2-antiovalbumin antibodies. However, different electrophoretic patterns of the C14-labelled 7 S antibodies were obtained from materials from different sources.
2. Furthermore, in all tissues examined, it was found that 7S γ1-globulin fractions had higher antiovalbumin antibody contents than 7S γ2-globulin fractions.
3. From these results, it was suggested that guinea pig 7S γ1- and 7S γ2-antibodies are synthesized by different types of plasma cells and also that electrophoretically different 7S γ2-antibodies are synthesized by different cells.
The present authors wish to express their sincere thanks to Prof. I. Yamashina for his encouragement during this work.
