Changes in Deoxyribonucleoprotein and RNA Polymerase Activity in Escherichia coli Infected with T2 Bacteriophage
1967 年 61 巻 5 号 p. 647-653
詳細
1967 年 61 巻 5 号 p. 647-653
Changes induced by T2 phage infection in the deoxyribonucleoprotein of Escherichia coli was studied, and rapid decreases in the protein content and DNA-dependent RNA polymerase [EC 2. 7. 7. 6] activity in the deoxyribonucleoprotein were observed.
The activity of host DNA as a primer for RNA polymerase was not altered at least 10 minutes after the phage infection. No restoration of the reduced level of the enzyme activity was achieved by purification of the enzyme. It was suggested that immediately after phage infection, a part of RNA polymerase in the deoxyribonucleoprotein was released together with the other proteins and the liberated enzyme was rapidly inactivated by degradation.
