Repressive Effect of Cortisone Administration on Protein Synthesis of Mouse Liver in vitro

抄録

1. In adrenalectomized mice, 30 and 60min after administration of cortisone, protein synthesis in the liver increased significantly, and heavy polysomes were formed similar to those in mice which had not received an operation.
2. At 3, 4 or 5 hr after its administration, cortisone caused marked repression of protein synthesis in mouse liver. The repressive factor(s) was present in both the polysomal fraction and the S-105 fluid. The amount of heavy polysomes did not decrease at this stage, judging from results of sucrose gradient analysis of the polysome distribution. The repressive effect of the S-105 fluid was larger than that of the polysomal fraction and so the effect may be called a “cytoplasmic repression.”
3. The earlier and later effects and the time course of the response to cortisone were similar in adrenalectomized and untreated mice.