Receptor Substance for Pyocin R

I. Partial Purification and Chemical Properties

Abstract

1. Pyocin R receptor substance was prepared from Pseudomonas aeruginosa P11 (pyocin R sensitive strain) by trichloroacetic acid extraction, nuclease digestion and pronase digestion. Most of the receptor substance was precipitated by ultracentrifugation.
2. This substance was mainly composed of lipopolysaccharide and contained glucose, rhamnose, glucosamine, galactosamine, heptose and a sugar giving a positive reaction with thiobarbituric acid in the carbohydrate moiety. It contained a small amount of amino acids.
3. The receptor activity decreased on chloroform-methanol extraction of the lipid, on alkali treatment or on periodate oxidation.
4. The TCA extract from the pyocin R resistant mutant (P11 R^r) had no receptor activity and no corresponding precipitates could be obtained from the extract by ultra-centrifugation.
5. The TCA extraction method was more effective than the phenol method for preparation of lipopolysaccharide with receptor activity.