Isolation and Characterization of the Antigenically Distinct Proteinpolysaccharide Complex from Human Aorta

抄録

The antibodies, formed in rabbits after the administration of the saline-soluble substances or crude proteinpolysaccharides from human aorta, were found to be those directed toward a glycoprotein which possessed chemical and physicochemical natures similar to those of the so-called bridging protein.
This antigenic component was eluted from DEAE-cellulose column in a relatively high quantity at the same elution volume as hyaluronic acid, migrated on electrophoresis as a diffuse band extending from α₂-globulin to prealbumin zone and filtered in a void volume of Sephadex G-200 column. The antigenic component existed in a form of macromolecular aggregate of proteinpolysaccharide complexes. After the hyaluronidase [EC 3. 2. 1. 35] digestion, the antigenically reactive moiety of this complex was isolated as a glucose-containing glycoprotein with the same order of dimension as albumin.