Cell-surface Changes of Phosphate-deficient Escherichia coli as Characterized by Increased Stability and Decreased Concanavalin A-Agglutinability of the Spheroplast
1974 年 76 巻 1 号 p. 65-72
詳細
1974 年 76 巻 1 号 p. 65-72
During growth under phosphate-deficient conditions, Escherichia coli K12: W3110 cells acquired an apparent resistance to hypotonic lysis accompanied by a markedly reduced release of perienzymes into the medium when subjected to spheroplast formation or “osmotic shock” treatment. In parallel with these changes in the cell membrane, a reduction in the agglutinability of phosphate-deficient spheroplasts was found when they were incubated with concanavalin A after trypsin treatment. Based on these results, cell surface changes caused by phosphate deficiency were discussed in relation to the known changes of cellular physiology, suggesting the usefulness of concanavalin A-specific agglutination as a probe for the study of cell membrane topography.
