Biosynthesis of Liver Catalase in Rats Treated with Allylisopropylacetylcarbamide

I. Immunochemical Assay of Catalase in Liver Cell Fractions

抄録

Rats were injected twice intraperitoneally with 20mg of allylisopropylacetylcarbamide (Sedormid) per 100g of body weight at an interval of 12hr. The level of catalase [EC 1. 11. 1. 6] in various liver cell fractions was determined both enzymatically and immunochemically 12 hr after the second injection.
1. The decrease in catalase protein assayed by the immunochemical method directly confirmed the inhibition of biosynthesis of the enzyme by this porphyrinogenic drug.
2. The occurrence of a considerable amount of catalase protein with no enzymatic activity was demonstrated both in the peroxisomes and in the supernatant fraction.
3. The amount of catalase-synthesizing polysomes in hepatic cell was reduced in Sedormid-treated rats by the extent comparable to the decrease in the concentration of liver catalase.