A Simple Fluorometric Assay for Type B Monoamine Oxidase Activity in Rat Tissues
1976 年 79 巻 6 号 p. 1297-1299
詳細
1976 年 79 巻 6 号 p. 1297-1299
A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4] activity towards β-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10mg to 100mg of liver tissue in 3ml of reaction mixture for up to 90min of incubation. Using this method, the Vmax values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).
