Roles of Nucleoside Triphosphates in Microtubule Assembly
1976 年 79 巻 6 号 p. 1357-1364
詳細
1976 年 79 巻 6 号 p. 1357-1364
Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35° with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruvate and pyruvate kinase [EC 2.7.1.40] also induced polymerization. Depolymerization of microtubules in the GTP-reassembly buffer yielded GTPETNGTP, which was capable of polymerizing into microtubules even in the absence of free GTP. In the presence of 4M glycerol, GDPETNGTP assembled into microtubules with no change in the bound nucleotides.
