The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
The Biosynthesis of Transfer RNA in Insects
II. Isolation of Transfer RNA Precursors from the Posterior Silk Gland of Bombyx mori
Ken-ichi TSUTSUMIReiko MAJIMAKensuke SHIMURA
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1976 年 80 巻 5 号 p. 1039-1045

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The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis.
Analysis of the incorporation of the methyl group from [methyl-14C] methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4, 5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.
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© The Japanese Biochemical Society
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