Cooperation of Cytochalasin D and Anti-Microtubular Agents in Stimulating Superoxide Anion Production in Polymorphonuclear Leukocytes

Abstract

Superoxide anion production in polymorphonuclear leukocytes obtained from guinea pig peritoneal cavities was stimulated by treatment with 5 μg/ml of cytochalasin D in vitro. The stimulation was further enhanced by the addition of either 10^-5-10^-7M vinblastine or 10^-3-10^-6M colchicine to cytochalasin D. Higher concentrations of these anti-microtubular agents were inhibitory to the stimulating effect of cytochalasin D on the superoxide anion production, while lower concentrations had almost no effect. In the resting leukocytes, i.e. without cytochalasin D, the anti-microtubular agents by themselves had no effect on the superoxide anion production. The cooperation of cytochalasin D and the anti-microtubular agents was also observed in the stimulation of NADPH oxidase activity and bactericidal function of the leukocytes.