Biosynthesis of Polymyxin E by a Cell-Free Enzyme System
II. Synthesis of Enzyme-Bound Octanoyldiaminobutyric Acid
1980 年 88 巻 1 号 p. 285-288
詳細
1980 年 88 巻 1 号 p. 285-288
A partially purified enzyme of Aerobacillus polyaerogenes, which produces polymyxin E, activates L-2, 4-diaminobutyric acid and binds it as a thioester. The incubation of the enzyme preparation with octanoyl coenzyme A and L-2, 4-diaminobutyric acid in the presence of ATP and an ammonium sulfate fraction yields octanoyldiaminobutyric acid thioesterified to the enzyme.
