The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
A Simple Method for the Determination of Glucosamine and Galactosamine Using Cellulose Acetate Electrophoresis
Itaru MIYAMOTOSumi NAGASE
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1982 Volume 91 Issue 4 Pages 1445-1447

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Abstract
A new electrophoretic method is presented for the determination of glucosamine and galactosamine. The technique is quite simple and rapid, it involves cellulose acetate electrophoresis in borate buffer (200 V, 15min) and silver nitrate staining. Hexosamine samples of 0.36-1.80 μg were separated and stained within 30min. This method was applied for the hexosamine analysis of glycosaminoglycans.
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© The Japanese Biochemical Society
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