Protuberic Acid-Hydrolytic Enzyme in Kobayasia nipponica and Characterization of the Hydrolytic Products

抄録

When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37°C and pH 4-5 in 0.1M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by ¹H- and ¹³C-NMR spectroscopy. They were identified as D-GIcUA and O-(α-L-IdUAp)-(1-4)-D-GIcUA. These results suggest that PAhydrolytic enzyme (s) include at least endo-β-D-glucuronidase.