抄録
Squid liver contains two kinds of α-N-acetylgalactosaminidases, which could be separated by gel filtration on Sephadex G-200 or by SP-Sephadex ion exchange chromatography. The two α-N-acetylgalactosaminidases, α-N-acetylgalactosaminidase I and II, were purified by procedures involving extraction, ammonium sulfate precipitation, and chromatographies on SP-Sephadex, Sephadex G-100, Sephadex G-200, DEAE-Sephadex, and Sepharose 6 B. Enzyme I was purified 1, 100-fold and enzyme II 3, 000-fold. Both enzymes appeared to be homogeneous based upon the results of disc gel electrophoresis. Enzyme I had a pH optimum of 3.0 and was heat-stable. It was inhibited by N-acetylgalactosamine and galactose. On the other hand, enzyme II had a pH optimum of 4.2 and was heat-labile. Galactose did not affect the enzyme activity. In contrast to enzyme I, which showed α-galactosidase activity even in the final preparation, enzyme II was practically free from α-galactosidase activity.
