Antibody against 25, 000 Dalton Tryptic Fragment of Subfragment-1 from Chicken Skeletal Muscle Myosin: Functional Implication of the 25, 000 Fragment Region in Subfragment-1

抄録

Antibody was prepared against the 25, 000-dalton tryptic fragment of subfragment-1 from skeletal muscle myosin. The antibody was found to inhibit the Mg²⁺-ATPase activity and the initial P₁-burst of the ATPase. The antibody suppressed the ATP-induced fluorescence enhancement of S-1, though it did not suppress the binding of ATP to S-1. The acto-S-1 ATPase activity was also inhibited by the antibody. These results suggest that there is a site in the 25K fragment region responsible for the transition of the myosin-ATP complex to another high energy complex.