A UV Laser-Scanning Confocal Microscope with a Hybrid Objective of Reflective and Refractive Elements

抄録

An optical system suitable for ultraviolet (UV) laser-scanning confocal microscopy was designed and custom-made. The optical system consisted of a hybrid objective of reflective and refractive elements (achromatic for 200—500 nm), a relay lens (200—500 nm) and a conventional confocal laser-scanning head. The system yielded lateral and axial resolutions of 0.6 μm and 3.5 μm, respectively, which were measured at a half decay length of the fluorescence of a bead (0.5 μm), and demonstrated the sliced image of cultured bullfrog sympathetic ganglion cells loaded with a Ca²⁺-sensitive fluorescent dye, lndo-1.