Effects of Low-intensity pulsed ultrasound (LIPUS) on osteogenic differentiation of murine periosteum-derived cells

Wai Myo Maung; Hidemi Nakata; Munemitsu Miyasaka; You-kyoung Kim; Motoi Miura; Maiko amamoto; Shohei Kasugai; Shinji Kuroda

doi:10.32176/biointeg.10.1_47

Wai Myo Maung, Hidemi Nakata, Munemitsu Miyasaka, You-kyoung Kim, Motoi Miura, Maiko amamoto, Shohei Kasugai, Shinji Kuroda

著者情報

Wai Myo Maung
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Hidemi Nakata
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Munemitsu Miyasaka
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
You-kyoung Kim
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Motoi Miura
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Maiko amamoto
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Shohei Kasugai
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Shinji Kuroda
Oral Implantology and Regenerative Dental Medicine, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,

キーワード: Low-intensity pulsed ultrasound, LIPUS, periosteum, gelatin, osteogenic Differentiation

ジャーナルオープンアクセス

2020 年 10 巻 1 号 p. 47-60

DOI https://doi.org/10.32176/biointeg.10.1_47

詳細

抄録

The replacement of lost natural teeth using osseointegrated implants require adequate bone volume permitting their anchorage. The periosteum is a specialized fibrous tissue composed of fibroblast, osteoblast, and progenitor cells and optimal source for bone tissue engineering based on its accessibility and rapid proliferation in vivo and in vitro. Low-intensity pulsed ultrasound (LIPUS) has shown successful bone healing of delayed, non-unions and fracture healing in animal models and in clinical treatments. This study aimed to investigate the effects of LIPUS on the proliferation and osteogenic differentiation of murine calvarial periosteum-derived cells (PDCs). PDCs were treated with daily ultrasound stimulation with an intensity of 30 mW/cm2, frequency of 3 MHz, pulse repetition frequency (PRF) of 1 kHz and exposure time of 20 min per day up to 21 days. The cell proliferation and viability were analyzed with PrestoBlue Cell Viability assay. Osteogenic differentiation was determined with alkaline phosphatase (ALP) staining, ALP activity assay, alizarin red staining, and real-time reverse transcriptase- polymerase chain reaction. The results indicated that LIPUS stimulation did not significantly affect the viability of PDCs. LIPUS significantly increased ALP activity on day 7 of culture and markedly promoted the formation of mineralized nodules on day 21. The mRNA expression of ALP, RUNX2, OSX, and OCN was significantly up-regulated by LIPUS stimulation. Thus, LIPUS stimulation increases osteogenic differentiation of PDCs and is useful for potential bone regeneration.

責任著者(Corresponding author)