Abstract
A strontium apataite (SrAp) thin film was coated onto
a titanium substrate using radio frequency sputtering
technique. To increase crystallinity of the sputtered films, the
coated films were subjected to a hydrothermal treatment.
The crystallinity of the films was determined using X-ray
diffractometer (XRD). Cell reaction to the prepared film
was evaluated by measuring the cell attachment of human
osteosarcoma cell line Saos-2, alkaline phosphatase (ALP)
activity and osteocalcin (OCN) expression of primary rat
osteoblast. From XRD patterns, the as-sputtered SrAp film
had higher crystallinity than the as-sputtered HA film. In the
cell attachment measurement, the number of attached cells
on the SrAp film after the hydrothermal treatment (hyd-
SrAp) was higher than that of the as-sputtered SrAp film,
whereas, the number of attached cells showed no significant
difference between the hyd-SrAp film and the hyd-HA film
(the HA film after the hydrothermal treatment). In the cell
area, both of the hyd-HA film and the hyd-SrAp film, showed
the same cell area as titanium. The number of vinculin on
the hyd-SrAp film was larger than that of the hyd-HA film.
The ALP activity for the hyd-SrAp film was higher than
the sp-SrAp film, whereas, the ALP activity for the hyd-
SrAp film was lower than that for the hyd-HA film. In OCN
expression, the hyd-SrAp film showed the largest value in all
samples. These results indicated that the hyd-SrAp film had
a good cell attachment, and the osteoblast differentiation on
the hyd-SrAp film was comparable to the hyd-HA film after
14 days of culture.
